Aurora kinase C AURKC Functions in Mammalian Spermatogenesis

Aurora kinase C Symbol AURKC:
Aurora kinase C Synonyms:
AIE2, AIK3, ARK3, ARK-3, AurC, Aurora/IPL1/Eg2 protein 2, Aurora/IPL1-related kinase 3, aurora-C, Aurora kinase C, Aurora-related kinase 3, Serine/threonine-protein kinase 13, Serine/threonine-protein kinase aurora-C, STK1.

Aurora kinase C:
The Aurora kinases are cell cycle-regulatory serine-threonine kinases too suffer been heard implicated in the function of the centrosomes, kinetechores, chromosome dynamics, and cytokinesis. In comparison surrounded by additionally tissues, there are insane prices of expression of Aurora-B and -C in testis. What the respective roles in mammalian spermatogenesis are is an open question.

Here we describe the expression and distribution cases of the 3 kinases in mouse testis employing in situ hybridization and immunohistochemistry. Importantly, the localization of Aurora-B is tightly regulated for the duration of spermatogenesis, whereas Aurora-C expression seems to be testis specific. To address the function of Aurora-B in spermatogenesis, we own generated transgenic mice utilizing a pachytene-stage-specific promoter driving the expression of either wild-type Aurora-B or an inactive generate of the kinase.

Expression of the inactive Aurora-B possible outcome in abnormal spermatocytes, increased apoptosis, spermatogenic arrest, and subfertility defects. The function of Aurora-C may additionally be targeted in the Aurora-B transgenic mutants. To address the function of Aurora-C in testis, we generated Aurora-C knockout mice by homologous recombination. Remarkably, Aurora-C null mice got viable, yet the males had compromised fertility. Aurora-C mutant sperm score abnormalities the present included heterogenous chromatin condensation, loose acrosomes, and blunted heads. These findings suggest who Aurora-B and Aurora-C represent professional works in mammalian spermatogenesis.